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Enterobacteria

The family Enterobacteriaceae includes bacteria that are the habitat of the intestine as a whole. But not all live in the intestine entero.

These bacteria are rod. gram -, and peritrichous Asporigeni, that have five flagella.

are aerobic, catalase +, oxidase -, ferment various sugars among different strains of enterobacteria.

The main genera of Enterobacteria are

• Escherichia coli, Diners Nell 'Intestine


• coliforms, Citrobacter, Enterobacter, Klebsiella


• Salmonella, one of the most dangerous


• Shigella, one of the most dangerous


• Proteus, one of the most dangerous


• Yersinia

CLASSIFICATION

1, classifications, based on recognition of surface antigens using specific antibodies. The surface antigens are on the flagella (H antigen), on the wall (O antigen) and capsule (K antigen)

2 ° classifications, based on several tests on the cultivation and fermentation of sugar lactose

A1.) Growth on Mc Conkey Agar

-is a selective and differential medium used for isolation and presumptive identification of Enterobacteriaceae.

Has-selective agents such as bile salt and lactose as differential agent.

Lactose -: Colonies small and whitish pink with spumatura

Lactose + with low acidity: Colonies Pink

Lactose + with High Acidity: Deep red colonies

A2.) Growth on Eosin Methylene Blue Agar

-is a selective medium for the eosin, and differential for lactose.

Lactose -: Colonies small and colorless

Lactose + with low acidity: Colonies in light gray or brown crossed

Lactose + with strong acidity: metallic green colonies

A3.) Growth on Hektoen Enteric Agar

x-Ground isolation of shigella and salmonella.

-distinguish from Shigella and Salmonella and Escherichia coliform coli

Shigella and Salmonella: green colonies with black centers

Coliforms: Colonies yellowish

B1.) Growth on Lactose Broth

- is a test that allows detection of E. coli and other coliforms (+ lactose with gas production) separately from other bacteria.

-x is the use of differential Duruahm bell, this bell collects gas produced from the fermentation of lactose.

B2) Growth on SS Agar

-SS is going to Shigella and Salmonella

-distinguish Shigella and Salmonella

from other enteric bacteria Shigella

: Small colonies colorless and dull

Salmonella: transparent colonies with black centers

coliforms and other non-Enterobacteriaceae growing x the reduction of sulfate to sulfide

B3.) Growth on Citrate Agar

allows detection of the entero-bacteria that are content citrate ion as the sole source of carbon.

distinguishes

-Enterobacter (lactose +) from E. Coli.

-Contains all inorganic substances except citrate and bromothymol blue indicator that turns blue in alkaline pH.

-piling and is seeded to smear simple

x-incubated 24 hours at 37 ° C

-If the ground is blue, the test is positive, the bacteria grew and produced alkaline substances.

C.) Enterotube: it is a material that can sow the same bacteria on different culture media simultaneously, thus reducing the time of sowing, the materials used Efaco correct identification of 'entero ..

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BACTERIA BACTERIA BACTERIA Staphylococcus STEPTOCOCCHI

are bacteria belonging to the family and gender main streptococcaceae streptococcus, a very large genus that includes many species that are similar to each other for many metabolic characteristics and morphological but are very different for the habitat and the relationship with man.

streptococci are bacteria responsible for various diseases such as pneumonia, bronchitis, scalattina, caries, septicemia, urinary tract infections ecc.ecc.ecc.

All streptococci are gram + cocci arranged in chains or oval, asporigeni, and have no flagella. Are oxidase and catalase -, tolerate low oxygen. ferment the sugar and agar colony forming small.

CLASSIFICATION

1 Rating: based hemolytic activity of the bacterium, namely the ability to damage red blood cells. Alfa Haemolytic
: when bacteria break down red blood cells but not transformed into a compound of hemoglobin color green.

Beta Haemolytic: When bacteria break both the red blood cell hemoglobin

Haemolytic Range: When not change any of them.

* Streptococcus is more dangerous because the beta-hemolytic streptococcus Piogenes .*



2 ° The classification Lancefield, is based on the recognition of a particular antigen polysaccharide antigen C said this on the wall of strepto.La presence of this antigen indicates the danger. The most pathogenic streptococci are grouped into group A streptococci, the second most dangerous in group B streptococci and so on.

3 ° The classification of Sherman, combines all the data on the ratings mentioned above.

VIRIDANTI: Alpha hemolytic, often without antigen, diners in the mouth .. The viridanti Mutans streptococci are pathogens responsible for tooth decay.

pyogenic: Beta hemolytic, Group A streptococci, All-pathogenic S. pyogenes.

faecal Alpha, Beta, Gamma Emolitic, Group D streptococci, intestinal opportunistic pathogens

MILK: gamma hemolytic, Lancefield group N, often used in the food

PNEUMONIAE *: Causes pneumonia, Alpha hemolytic.





Practical Experiences


ACTIVITIES 'HAEMOLYTIC:

It grows on a soil bacterium called AZIDE blood agar, nutrient agar with added sheep blood, you incubated
x 24 hours 37 ° C, observes,

* X I've Sown The bacterium is a pathogenic bacterium because all'intor no colonies there is a double halo, which indicates that the bacteria have damaged red blood cells.

IDENTIFICATION OF THE BACTERIUM CONSIDER



after discovering that they are beta hemolytic, we sowed the same bacterium in another land to Striplosemplice Azide Blood Agar, we somesso of paper discs containing antibiotics that are known and optochin bacitrocina.



As you can see the bacteria is not sensitive to bacitrocina (above the disc) but is sensitive to 'optochin (under the disc) so we can see that the bacterium is a Fecalys.

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